Human MC2R knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human MC2R knockout HeLa cell lysate (AB258511)
Allele-1 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MC2R knockout HeLa cell lysate (AB258511)
Allele-2 : 1 bp insertion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The action of MC2-R initiates the adrenal response to stress through glucocorticoid production which is important for maintaining homeostasis in the body. MC2-R functions as a standalone entity rather than as part of a larger complex. By binding to ACTH MC2-R triggers downstream signaling that leads to steroidogenesis. The biosynthesis of glucocorticoids helps in managing energy immune function and circadian rhythm.
Pathways
MC2-R is integral in the cAMP signaling pathway and the stress response pathway. In the cAMP signaling activation of MC2-R by ACTH results in increased production of cyclic AMP further promoting enzyme activation essential for glucocorticoid synthesis. Its activity is connected to the broader steroid hormone biosynthesis pathway working closely with proteins like StAR (steroidogenic acute regulatory protein) which facilitates cholesterol transport to the inner mitochondrial membrane.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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