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AB258512

Human MCFD2 knockout HeLa cell lysate

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MCFD2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 182 bp deletion in exon4 and 1 bp insertion in exon4.
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Sanger Sequencing - Human MCFD2 knockout HeLa cell lysate (AB258512)
  • Sanger seq

Unknown

Sanger Sequencing - Human MCFD2 knockout HeLa cell lysate (AB258512)

Allele-1 : 182 bp deletion in exon4

Sanger Sequencing - Human MCFD2 knockout HeLa cell lysate (AB258512)
  • Sanger seq

Unknown

Sanger Sequencing - Human MCFD2 knockout HeLa cell lysate (AB258512)

Allele-2 : 1 bp insertion in exon4

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 182 bp deletion in exon4 and 1 bp insertion in exon4.

Disease

Adenocarcinoma

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Complementary Products

Proteins & Peptides

AB92930

Recombinant Human MCFD2 protein

SDS-PAGE - Recombinant Human MCFD2 protein (AB92930)

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Cell Lines & Lysates

AB265164

Human MCFD2 knockout HeLa cell line

Sanger Sequencing - Human MCFD2 knockout HeLa cell line (AB265164)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MCFD2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCFD2 also known as Multiple Coagulation Factor Deficiency 2 operates as an important protein involved in transport processes within cells. This protein has an approximate mass of 16 kDa and is primarily expressed in the endoplasmic reticulum. MCFD2 partners with LMAN1 (lectin mannose-binding 1) to form a calcium-dependent complex that is essential for certain protein trafficking functions.
Biological function summary

Within cellular systems MCFD2 plays a significant role in the transportation of specific coagulation factors. By forming a complex with LMAN1 MCFD2 assists in packaging cargo proteins like Factor V and Factor VIII ensuring their proper movement from the endoplasmic reticulum to the Golgi apparatus. This function highlights its involvement in maintaining hemostatic balance.

Pathways

Protein trafficking and coagulation processes prominently feature MCFD2's activity. MCFD2 is actively involved in the secretory pathway which includes key proteins such as Factor V and Factor VIII. Its function within these pathways underlines the protein's importance in normal clotting mechanisms supporting overall circulatory health.

Malfunction or deficiency in MCFD2 directly links to Combined Factor V and Factor VIII deficiency (F5F8D). This rare bleeding disorder exemplifies an association where MCFD2's disruption affects coagulation factor transport leading to improper clot formation. The interplay with LMAN1 is also important as mutations in either component can result in similar coagulation defects highlighting a direct impact on blood clotting-related diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com