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AB263250

Human MEA1 knockout HEK-293T cell lysate

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MEA1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.
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Sanger Sequencing - Human MEA1 knockout HEK-293T cell lysate (AB263250)
  • Sanger seq

Unknown

Sanger Sequencing - Human MEA1 knockout HEK-293T cell lysate (AB263250)

Allele-2 : 14 bp insertion in exon 2

Sanger Sequencing - Human MEA1 knockout HEK-293T cell lysate (AB263250)
  • Sanger seq

Unknown

Sanger Sequencing - Human MEA1 knockout HEK-293T cell lysate (AB263250)

Allele-1 : 10 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.

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Complementary Products

Cell Lines & Lysates

AB266808

Human MEA1 knockout HEK-293T cell line

Sanger Sequencing - Human MEA1 knockout HEK-293T cell line (AB266808)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MEA1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEA1 also known as Male-enhanced antigen 1 is a protein that plays a significant role in the process of spermatogenesis. It is a protein composed of approximately 65 kDa. MEA1 is predominantly expressed in the testes indicating its involvement in male reproductive function. The protein has been identified to participate in cellular processes essential to germ cell development and its expression pattern suggests it may have functions that are testis-specific or testis-enhanced.
Biological function summary

MEA1 interacts with cellular machinery involved in cell cycle regulation and differentiation. Its role has been observed in the development of male germ cells. MEA1 does not form part of a larger protein complex; instead it functions as an independent entity that supports spermatid differentiation. It seems essential for proper morphological changes necessary for mature sperm formation.

Pathways

Studies show that MEA1 is integral to pathways guiding spermatogenesis and germ cell maturation. It does not directly link to more widespread signaling routes like MAPK or PI3K. MEA1 may interact with proteins such as CREM a transcription factor that regulates genes critical for sperm cell development suggesting it integrates into the transcriptional regulatory framework essential for efficient sperm production.

MEA1’s aberrant expression is associated with male infertility specifically non-obstructive azoospermia which is a condition requiring intervention for sperm production failures. Connections to infertility emphasize the protein's role in maintaining healthy sperm production. Another protein associated with male infertility is DAZL which MEA1 could potentially interact with given their shared involvement in spermatogenesis. Further research could elucidate MEA1’s exact interactions and pathogenic roles connected to reproductive issues.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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