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AB261653

Human MFN2 (Mitofusin 2) knockout HEK-293 cell lysate

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MFN2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 10 bp deletion.

View Alternative Names

CMT2A, CMT2A2, CPRP 1, EC 3.6.5.-, Fzo, HSG, Hypertension related protein 1, KIAA0214, MARF, MFN2_HUMAN, Mitochondrial assembly regulatory factor, Mitofusin-2, Transmembrane GTPase MFN2, hyperplasia suppressor gene

1 Images
Western blot - Human MFN2 (Mitofusin 2) knockout HEK-293 cell lysate (AB261653)
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Lab

Western blot - Human MFN2 (Mitofusin 2) knockout HEK-293 cell lysate (AB261653)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : MFN2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab205236 observed at 86 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab205236 was shown to recognize MFN2 (Mitofusin 2) in wild-type HEK-293 cells as signal was lost at the expected MW in MFN2 knockout cell line ab260861 (knockout cell lysate ab261653). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and MFN2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab205236 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Mitofusin 2 antibody [EPR19796] (<a href='/en-us/products/primary-antibodies/mitofusin-2-antibody-epr19796-ab205236'>ab205236</a>) at 1/2000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

MFN2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human MFN2 (Mitofusin 2) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-mfn2-mitofusin-2-knockout-hek-293-cell-line-ab260861'>ab260861</a>)

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 86 kDa

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Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 10 bp deletion

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MFN2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofusin 2 also known as MFN2 is a protein involved in the regulation of mitochondrial fusion. The MFN2 molecular weight is roughly 86 kDa. It plays an important role in connecting and merging the outer membranes of mitochondria which is vital for maintaining mitochondrial function and integrity. Mitofusin 2 proteins are expressed in many tissues but they are abundantly present in energy-demanding tissues like skeletal muscle heart and the brain.
Biological function summary

Mitofusin 2 ensures the proper distribution of mitochondria within cells and regulates mitochondrial metabolism. It is a critical component of the mitochondrial fusion machinery and works closely with its homolog Mitofusin 1 (MFN1). Together they form a complex that facilitates the physical merging of mitochondrial membranes. This process is essential for mitochondrial dynamics which include not only fusion but also fission and biogenesis.

Pathways

The protein part of the fusion machinery integrates into multiple essential biological pathways including energy metabolism and apoptosis regulation. It participates in the mitochondrial fusion pathway and the PGC-1α pathway for mitochondrial biogenesis. Mitofusin 2 interacts with proteins such as PINK1 and Parkin that are known to play roles in mitophagy a process that targets damaged mitochondria for degradation indicating its involvement in maintaining mitochondrial quality control.

Mutations in Mitofusin 2 have been linked to Charcot-Marie-Tooth disease type 2A (CMT2A) a neuropathy that affects peripheral nerves. This protein also shows connections to metabolic disorders such as obesity and type 2 diabetes. In these conditions its interaction with other proteins like OPA1 involved in mitochondrial inner membrane fusion influences mitochondrial dysfunction a recognized feature contributing to disease pathogenesis. Understanding MFN2's function and role in disease can help develop targeted therapies for these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB260861

Human MFN2 (Mitofusin 2) knockout HEK-293 cell line

Advanced Validation

Western blot - Human MFN2 (Mitofusin 2) knockout HEK-293 cell line (AB260861)

  • 0
  • 0
Primary Antibodies

AB124773

Anti-Mitofusin 2 antibody [NIAR164]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (AB124773)

  • 4
  • 6
Primary Antibodies

AB157457

Anti-OPA1 antibody [EPR11057(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (AB157457)

  • 4
  • 5
Primary Antibodies

AB126575

Anti-Mitofusin 1 antibody [11E91H12]

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (AB126575)

  • 5
  • 2

Product promise

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