Human MGEA5 (OGA) knockout HeLa cell lysate
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OGA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.
View Alternative Names
Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Beta-hexosaminidase, Bifunctional protein NCOAT, HEXC, Hyaluronidase in meningioma, KIAA0679, MEA5, MGEA 5, Meningioma-expressed antigen 5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, NCOAT, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA_HUMAN, Protein O-GlcNAcase
- WB
Unknown
Western blot - Human MGEA5 (OGA) knockout HeLa cell lysate (AB257263)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : MGEA5 knockout HeLa cell lysate (20 μg)
Lane 3 : JAR cell lysate (20 μg)
Lane 4 : 293T cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab124807 observed at 130 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab124807 Anti-MGEA5/OGA antibody [EPR7154(B)] was shown to specifically react with MGEA5/OGA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265821 (knockout cell lysate ab257263) was used. Wild-type and MGEA5/OGA knockout samples were subjected to SDS-PAGE. ab124807 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (<a href='/en-us/products/primary-antibodies/mgea5-oga-antibody-epr7154b-ab124807'>ab124807</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MGEA5 knockout HeLa cell lysate at 20 µg
Lane 3:
JAR cell lysate at 20 µg
Lane 4:
293T cell lysate at 20 µg
Predicted band size: 102 kDa
Observed band size: 130 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MGEA5 (OGA) knockout HeLa cell lysate (AB257263)
Homozygous : 2 bp insertion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MGEA5 acts as an important modulator of protein function and regulation by cycling O-GlcNAc modifications thereby influencing processes like transcription signal transduction and apoptosis. While not part of a well-defined complex it frequently interacts with other proteins involved in glucose metabolism and the cellular stress response. Its activity works in concert with OGT (O-GlcNAc transferase) which adds O-GlcNAc maintaining a balance important for cellular function.
Pathways
MGEA5 involvement centers largely around glucose homeostasis and the insulin signaling pathway. It modulates the function of proteins like insulin receptor substrate (IRS) and other key regulators in this pathway affecting metabolic regulation and energy homeostasis. It shows a direct relationship with OGT as they reciprocate in action effects highlighting their collaborative role in cellular pathways that affect cellular signaling and survival.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB124807
Anti-MGEA5/OGA antibody [EPR7154(B)]
RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (AB124807)](https://content.abcam.com/products/images/mgea5-oga-antibody-epr7154b-ab124807--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130024.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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