Human MISP (C19orf21) knockout HeLa cell lysate
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MISP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
View Alternative Names
MISP_HUMAN, Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein, Uncharacterized protein C19orf21, chromosome 19 open reading frame 21, hypothetical protein LOC126353
- Sanger seq
Unknown
Sanger Sequencing - Human MISP (C19orf21) knockout HeLa cell lysate (AB263252)
Allele-1 : 1 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human MISP (C19orf21) knockout HeLa cell lysate (AB263252)
Allele-2 : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C19orf21 plays a role in regulating cellular homeostasis and proliferation. C19orf21 is part of the chromatin remodeling complex which modifies chromatin to allow access to transcription machinery. This participation suggests importance in controlling gene accessibility. C19orf21's involvement in such intricate processes indicates a possible role in cellular development and differentiation influencing tissue-specific gene expression.
Pathways
C19orf21 is involved in chromatin modification and transcriptional regulation pathways. It interacts with proteins like histone acetyltransferases contributing to epigenetic modifications and regulation of gene expression. Additionally C19orf21 shows involvement in pathways that maintain DNA integrity working alongside proteins like DNA repair enzymes. These interactions suggest that C19orf21 supports cellular responses to damage and stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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