Human MKI67 (Ki67) knockout HeLa cell lysate
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MKI67 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 7.
View Alternative Names
Antigen KI-67, Antigen identified by monoclonal antibody Ki-67, KI67_HUMAN, KIA, MKI67, Marker of proliferation Ki-67, PPP1R105, Proliferation marker protein Ki-67, Proliferation related Ki 67 antigen, Protein phosphatase 1 regulatory subunit 105, RP11-380J17.2
- Sanger seq
Unknown
Sanger Sequencing - Human MKI67 (Ki67) knockout HeLa cell lysate (AB263762)
Homozygous : 7 bp deletion in exon 7
- WB
Lab
Western blot - Human MKI67 (Ki67) knockout HeLa cell lysate (AB263762)
Lane 1 : Ramos cell lysate (20 μg)
Lane 2 : Wild-type HeLa cell lysate (20 μg)
Lane 3 : MKI67 knockout HeLa cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab16667 observed at 359 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab16667 was shown to react with Ki67 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255407 (knockout cell lysate ab263762) was used. Wild-type and Ki67 knockout samples were subjected to SDS-PAGE. ab16667 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 100 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Ki67 antibody [SP6] (<a href='/en-us/products/primary-antibodies/ki67-antibody-sp6-ab16667'>ab16667</a>) at 1/100 dilution
Lane 1:
Ramos cell lysate at 20 µg
Lane 2:
Wild-type HeLa cell lysate at 20 µg
Lane 3:
Western blot - Human MKI67 (Ki67) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mki67-ki67-knockout-hela-cell-line-ab255407'>ab255407</a>) at 20 µg
Lane 3:
Western blot - Human MKI67 (Ki67) knockout HeLa cell lysate (ab263762) at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 358 kDa
Observed band size: 124 kDa,359 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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