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AB258516

Human MKNK1 (MNK1) knockout HeLa cell lysate

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MKNK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
3 Images
Western blot - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)
  • WB

Lab

Western blot - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)

All lanes:

Anti-MKNK1 antibody at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 1/20 dilution

Lane 2:

Western blot - Human MKNK1 (MNK1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mknk1-mnk1-knockout-hela-cell-line-ab264758'>ab264758</a>)

Lane 2:

Western blot - Human MKNK1 (MNK1) knockout HeLa cell lysate (ab258516)

Lane 3:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 50 kDa

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Sanger Sequencing - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)
  • Sanger seq

Unknown

Sanger Sequencing - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)

Allele-1 : 1 bp deletion in exon2

Sanger Sequencing - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)
  • Sanger seq

Unknown

Sanger Sequencing - Human MKNK1 (MNK1) knockout HeLa cell lysate (AB258516)

Allele-2 : Insertion of the selection cassette in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB76256

Anti-eIF4E (phospho S209) antibody [EP2151Y]

RabMAb|Recombinant

Western blot - Anti-eIF4E (phospho S209) antibody [EP2151Y] (AB76256)

  • 5
  • 2
Cell Lines & Lysates

AB264704

Human MOK knockout HeLa cell line

Sanger Sequencing - Human MOK knockout HeLa cell line (AB264704)

  • 0
  • 0
Proteins & Peptides

AB125635

Recombinant human MNK1 protein

Functional Studies - Recombinant human MNK1 protein (AB125635)

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  • 0
Biochemicals

AB141267

PD 158780, ErbB receptor tyrosine kinase inhibitor

Chemical Structure - PD 158780, ErbB receptor tyrosine kinase inhibitor (AB141267)

  • 0
  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MKNK1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitogen-activated protein kinase-interacting serine/threonine-protein kinase 1 (MNK1) also known as MAP kinase-interacting kinase 1 is an enzyme with a molecular weight of approximately 51 kDa. MNK1 plays an important role in the regulation of protein synthesis by phosphorylating eukaryotic initiation factor 4E (eIF4E). Researchers find high expression of MNK1 in many tissues especially within the brain and immune system which suggests its involvement in diverse cellular processes.
Biological function summary

The protein kinase MNK1 regulates the translation of mRNA by forming part of the signaling complex that includes eIF4E. This complex plays a role in stress response cell growth and the immune response. Protein synthesis regulated by MNK1 affects cellular activities by selectively translating specific mRNAs related to those processes. MNK1's specific regulation often depends on signals received through upstream kinases.

Pathways

MNK1 is a part of the mitogen-activated protein kinase (MAPK) signaling cascade particularly the extracellular signal-regulated kinase (ERK) and p38 MAPK pathways. These pathways find involvement in transmitting signals from the cell membrane to the nucleus affecting gene expression and cellular responses. MNK1 modulates its activity in response to signals from upstream proteins such as ERK1/2 or p38α/β which are likely to influence the regulation of eIF4E and therefore translation initiation.

Scientists observe MNK1 implications in cancer progression and neurodegenerative diseases. MNK1-mediated phosphorylation of proteins like eIF4E and its overactivation often correlates with tumor growth and resistance to chemotherapy. Additionally research links MNK1's altered activity to neurodegenerative conditions such as Alzheimer's disease. Interactions between MNK1 and other proteins like eIF4E suggest potential roles for MNK1 in these disease pathologies making it an attractive target for therapeutic interventions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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