Human MKNK2 (MNK2) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human MKNK2 (MNK2) knockout HEK-293T cell lysate (AB258517)
Allele-2 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MKNK2 (MNK2) knockout HEK-293T cell lysate (AB258517)
Allele-1 : 20 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MNK2 modulates stress responses by influencing protein synthesis. It is an integral component of the mRNA translation machinery. MNK2 phosphorylates the eukaryotic translation initiation factor 4E (eIF4E) a process that is fundamental for the translation of specific mRNAs. By regulating eIF4E activity MNK2 helps to control the translation of mRNAs involved in stress response and cellular growth.
Pathways
MNK2 is significantly involved in the MAPK signaling pathway and plays a role in the response to cellular stress and growth signals. This pathway includes MAP kinases like ERK which activate MNK2. Through its interaction with eIF4E MNK2 facilitates the synthesis of proteins that are important for cell survival and proliferation in response to extracellular signals. Its action links upstream signaling events influenced by MAP kinases to downstream processes affecting cell growth and stress response.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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