Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell lysate
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(1 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell lysate (AB258965)
Homozygous : 1 bp insertion in exon2
Complementary Products
Cell Lines & Lysates
AB266868
Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell line
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GBL contributes to the regulation and setting up of signal transduction pathways by forming a part of the G protein complex. This complex involves the alpha beta and gamma subunits where GBL typically interacts with the beta subunit family. By binding to receptors like GPCRs GBL facilitates the exchange of GDP for GTP on the G protein alpha subunit thereby activating downstream effectors. The complex is critical in ensuring precise cellular responses and GBL maintains structural integrity within it.
Pathways
GBL plays an integral role in the G protein-coupled receptor (GPCR) signaling and the MAPK signaling pathways. In GPCR signaling GBL's interaction with the beta subunit influences processes like sensory perception and cell growth. In the MAPK pathway critical for cell responses to growth signals GBL connects with proteins such as RAF and MEK supporting the cascade of phosphorylation events critical for pathway progression. These pathways highlight GBL's importance in cellular dynamics and network interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Molecular medicine reports 18:1571-1581 PubMed29901205
2018
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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