Human MMP24 knockout HeLa cell lysate
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MMP24 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon2 and 8 bp deletion in exon2.
View Alternative Names
MMP24_HUMAN, MT-MMP 5, MT5-MMP, Matrix metallopeptidase 24, Matrix metallopeptidase 24 (membrane inserted), Membrane-type matrix metalloproteinase 5, Membrane-type-5 matrix metalloproteinase, Processed matrix metalloproteinase-24
- Sanger seq
Unknown
Sanger Sequencing - Human MMP24 knockout HeLa cell lysate (AB258966)
Allele-1 : 25 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MMP24 knockout HeLa cell lysate (AB258966)
Allele-2 : 8 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme is involved in the modulation of synaptic connections and neural plasticity. MMP24 functions as part of a complex which includes pro-protein convertases that activate the enzyme from its pro-form. These activities highlight its contribution to the regulation of neural networks. MMP24's interaction with neuronal substrates and their subsequent processing is vital for maintaining normal neural architecture and response to recovery processes after injury.
Pathways
MMP24 holds a significant role in the extracellular matrix (ECM) remodeling pathway and is linked to the integrin signaling pathway. These pathways are important for cell adhesion migration and survival. In the ECM remodeling pathway MMP24 interacts with other metalloproteinases such as MMP2 and MMP9 highlighting its contribution to the dynamic remodeling processes. In the integrin signaling pathway its activity affects cell-matrix interactions emphasizing its importance in cellular communication and structural organization within tissues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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