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AB258967

Human MMRN1 (ECM) knockout HeLa cell lysate

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MMRN1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.

View Alternative Names

Elastin microfibril interface located protein 4, MMRN, Endothelial cell multimerin, EMILIN4, Elastin microfibril interfacer 4, EMILIN-4, MMRN1, GPIA*, ECM, Multimerin-1

3 Images
Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)
  • Sanger seq

Unknown

Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)

Allele-3 : Insertion of the selection cassette in exon1

Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)
  • Sanger seq

Unknown

Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)

Allele-1 : 1 bp deletion in exon1

Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)
  • Sanger seq

Unknown

Sanger Sequencing - Human MMRN1 (ECM) knockout HeLa cell lysate (AB258967)

Allele-2 : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MMRN1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The extracellular matrix (ECM) constitutes a complex network of macromolecules such as proteins and glycoproteins including the key structural proteins like collagen elastin fibronectin and laminin. It provides structural support to cells and tissues and plays a role in signal transmission across the cellular environment. The ECM does not have a singular mass due to its diverse composition but is expressed throughout different tissues in the body. Its complexity and vast distribution are essential for maintaining tissue architecture and function.
Biological function summary

The extracellular matrix coordinates cellular functions by mediating mechanical and biochemical signals influencing cellular processes such as adhesion differentiation and migration. The ECM is an essential component of the matrix receptor complex interacting dynamically with cell-surface receptors like integrins. These interactions are important to tissue remodeling and repair allowing tissues to maintain homeostasis and respond to external stresses.

Pathways

The extracellular matrix participates in significant biological pathways like the integrin signaling pathway and the Wnt signaling pathway. These pathways link ECM to various cellular responses affecting processes such as cell migration proliferation and survival. Integrin proteins for example bind to ECM proteins transmitting signals that can regulate gene expression and determine cell fate. Disruption in these pathways has implications for developmental processes and disease states.

The extracellular matrix plays a role in conditions such as fibrosis and cancer. Alterations in ECM components or remodelling can lead to fibrotic diseases where excessive ECM deposition affects tissue function. In cancer ECM changes can influence tumor progression and metastasis. Matrix metalloproteinases (MMPs) a group of enzymes breaking down ECM proteins connect ECM to these pathological states altering tissue integrity and facilitating disease progression. Understanding ECM dynamics is therefore essential for developing therapeutic strategies against these disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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