MPDU1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Alternative names
CDGIF, LEC35, MPDU1, MPU1_HUMAN, Mannose-P-dolichol utilization defect 1 protein, PQLC5, SL15, Suppressor of Lec15 and Lec35 glycosylation mutation homolog, mannose P dolichol utilization defect 1
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MPDU1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
- Concentration
Properties
- Gene name
- MPDU1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SL15 also known as Signal Ligand 15 is an important protein involved in cellular signaling. It exhibits a molecular mass around 58 kDa and shows widespread expression in immune cells particularly in macrophages and dendritic cells. The protein consists of several domains that aid in its role in transmission of signals across the cell membrane triggering intracellular responses. Its expression is highest in tissues involved in immune responses such as the spleen and lymph nodes where it plays a role in the modulation of immune functions.
- Biological function summary
SL15 functions as a pivotal element in initiating immune cell activation. It is a component of the SL15-Complex which interacts with other receptors on the cell surface to mediate downstream signaling cascades. This interaction can lead to changes in gene expression that promote inflammatory responses. Working in tandem with co-receptors SL15 plays a central role in regulating the intensity and duration of immune reactions which are critical for maintaining homeostasis and defense against pathogens.
- Pathways
SL15 is essential in the regulation of the NF-kB signaling pathway which is vital for immune and stress responses. Its involvement in this pathway connects it to a network of other proteins such as the TRAF family that facilitate the NF-kB transcriptional activation. SL15 also participates in the MAPK signaling pathway where it influences cell proliferation and differentiation. These pathways ensure that the cell responds appropriately to external stimuli and maintain cellular balance.
- Associated diseases and disorders
SL15 has garnered interest due to its association with autoimmune diseases like rheumatoid arthritis and inflammatory bowel disease. Abnormal SL15 signaling may result in improper immune responses leading to chronic inflammation. It shows interactions with proteins like IL-6 and TNF-alpha which are mediators of inflammation and have been implicated in disease progression. Targeting SL15 in therapeutic interventions might offer new avenues for managing these conditions.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human MPDU1 (SL15) knockout HEK-293T cell lysate (ab258968)
Allele-1: 2 bp deletion in exon 1
Sanger Sequencing - Human MPDU1 (SL15) knockout HEK-293T cell lysate (ab258968)
Allele-2: Insertion of the selection cassette in exon 1
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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