Human MPDU1 (SL15) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human MPDU1 (SL15) knockout HEK-293T cell lysate (AB258968)
Allele-1 : 2 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human MPDU1 (SL15) knockout HEK-293T cell lysate (AB258968)
Allele-2 : Insertion of the selection cassette in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SL15 functions as a pivotal element in initiating immune cell activation. It is a component of the SL15-Complex which interacts with other receptors on the cell surface to mediate downstream signaling cascades. This interaction can lead to changes in gene expression that promote inflammatory responses. Working in tandem with co-receptors SL15 plays a central role in regulating the intensity and duration of immune reactions which are critical for maintaining homeostasis and defense against pathogens.
Pathways
SL15 is essential in the regulation of the NF-kB signaling pathway which is vital for immune and stress responses. Its involvement in this pathway connects it to a network of other proteins such as the TRAF family that facilitate the NF-kB transcriptional activation. SL15 also participates in the MAPK signaling pathway where it influences cell proliferation and differentiation. These pathways ensure that the cell responds appropriately to external stimuli and maintain cellular balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com