Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate
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MPI KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 3.
View Alternative Names
CDG1B, FLJ39201, MANNOSEPHOSPHATE ISOMERASE, MGC94106, MPI_HUMAN, Mannose-6-phosphate isomerase, PMI, PMI1, Phosphohexomutase, Phosphomannose isomerase, phosphomannose isomerase 1
- WB
Lab
Western blot - Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate (AB258523)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : Raji cell lysate 20 μg
False colour image of Western blot : Anti-MPI antibody staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to MPI. A band was observed at 47 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MPI knockout cell line ab266298 (knockout cell lysate ab258523). To generate this image, wild-type and MPI knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate (AB258523)
Homozygous : 2 bp insertion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
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Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme facilitates the processing of mannose through the biosynthesis pathways of glycoproteins and glycolipids. Mannose Phosphate Isomerase is not part of a larger protein complex but it functions independently to execute its role within the cell. It ensures that cells have adequate mannose-6-phosphate which is essential for proper glycosylation a process necessary for protein folding and stability.
Pathways
Mannose Phosphate Isomerase influences the hexosamine biosynthesis pathway and glycolysis. In the hexosamine biosynthesis pathway it works closely with enzymes such as phosphomannomutase to provide critical components for the production of glycosaminoglycans and glycoproteins. In glycolysis it helps integrate mannose into energy production processes by providing intermediates necessary for glucose metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266298
Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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