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AB258050

Human MPLKIP (TTDN1) knockout HeLa cell lysate

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MPLKIP KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
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Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)
  • Sanger seq

Unknown

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)

Allele-2 : 1 bp deletion in exon 1

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)
  • Sanger seq

Unknown

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)

Allele-1 : Insertion of the selection cassette in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB264878

Human MPLKIP (TTDN1) knockout HeLa cell line

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell line (AB264878)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MPLKIP
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTDN1 also known as C20orf174 is a protein involved in cellular processes that facilitate DNA repair. It weighs approximately 47 kilodaltons and is expressed in various tissues with higher levels observed in testis and ovary. The protein plays an essential role in maintaining genomic integrity by interacting with specific components of the DNA repair machinery.
Biological function summary

TTDN1 contributes to the repair of double-strand DNA breaks. It forms part of the larger DNA repair complex which recruits and coordinates other proteins needed for effective DNA repair. This function is especially important in cells exposed to frequent DNA damage supporting cellular stability and proper functioning by ensuring accurate DNA replication and transmission.

Pathways

The functions of TTDN1 intersect with the homologous recombination and non-homologous end joining pathways. It interacts with proteins such as BRCA1 and RAD51 which are key in DNA damage response and repair. These pathways enable cells to prevent mutations therefore safeguarding the genome's integrity during cell division and proliferation.

TTDN1 is associated with trichothiodystrophy a condition characterized by defects in DNA repair mechanisms. This association suggests TTDN1's involvement in clinical presentations linked to DNA repair deficiencies. Additionally anomalies in proteins like ERCC2 which also participates in DNA repair may exacerbate the effects when TTDN1 function is impaired leading to more severe disease outcomes.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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