Human MPP1 knockout HEK-293T cell lysate
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MPP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon6.
View Alternative Names
55 kDa erythrocyte membrane protein, AAG 12, Aging associated gene 12, DXS552, DXS552E, EM55_HUMAN, EMP 55, Erythrocyte membrane protein p55, MRG-1, Membrane protein, Membrane protein palmitoylated 1, Membrane protein palmitoylated 1 55kDa, Migration related gene 1, OTTHUMP00000026054, OTTHUMP00000196176, PEMP, Palmitoylated erythrocyte membrane protein, Palmitoylated membrane protein 1, p55, palmitoylated 1
- WB
Unknown
Western blot - Human MPP1 knockout HEK-293T cell lysate (AB258051)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : MPP1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : K-562 cell lysate (20 ug)
ab108528 was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab108528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- WB
Unknown
Western blot - Human MPP1 knockout HEK-293T cell lysate (AB258051)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : MPP1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : K-562 cell lysate (20 ug)
ab133500 was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab133500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human MPP1 knockout HEK-293T cell lysate (AB258051)
Homozygous : 13 bp deletion in exon6
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.
Pathways
Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Primary Antibodies
AB216347
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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