Human MPRIP knockout HeLa cell lysate
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MPRIP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and Insertion of the selection cassette in exon4.
View Alternative Names
9530046C02, AA536749, AI647711, C76423, KIAA0864, M-RIP, MGC67316, MPRIP_HUMAN, Myosin phosphatase Rho-interacting protein, RP23-180B18.4, Rho-interacting protein 3, Rhoip3, mKIAA0864, p116 Rho-interacting protein, p116Rip
- Sanger seq
Unknown
Sanger Sequencing - Human MPRIP knockout HeLa cell lysate (AB263255)
Allele-1 : Insertion of the selection cassette in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human MPRIP knockout HeLa cell lysate (AB263255)
Allele-2 : 1 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MPRIP serves a critical role in the modulation of actin cytoskeleton rearrangement. It is part of a multi-protein complex that includes myosin phosphatase which is essential for dephosphorylation of myosin light chains. By binding directly to the myosin phosphatase complex MPRIP ensures proper regulation of actin-myosin interactions which are integral to cellular processes such as migration division and shape maintenance.
Pathways
MPRIP plays an important role within the RhoA signaling pathway and the regulation of cytoskeletal rearrangements. It facilitates the interaction between RhoA and myosin phosphatase leading to the deactivation of contractility. Another important protein involved is ROCK as MPRIP counteracts ROCK-induced phosphorylation of myosin light chain therefore influencing cell contractility and motility.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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