Human MPV17 knockout HeLa cell lysate
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MPV17 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.
View Alternative Names
Glomerulosclerosis, MPV17_HUMAN, MTDPS6, MpV17 mitochondrial inner membrane protein, Mpv17 human homolog of glomerulosclerosis and nephrotic syndrome, Protein Mpv17, SYM1
- Sanger seq
Unknown
Sanger Sequencing - Human MPV17 knockout HeLa cell lysate (AB258970)
Homozygous : 1 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MPV17 affects several processes related to cellular energy production. It is part of a larger protein family involved in mitochondrial homeostasis. MPV17 contributes to the regulation of oxidative phosphorylation by modulating mitochondrial DNA content. This regulation assures proper function of the respiratory chain complexes required for cellular energy provision. Additionally its activity helps prevent mtDNA damage that may arise from oxidative stress.
Pathways
MPV17 influences energy metabolism pathways critical for cell viability. It interacts with the mitochondrial DNA maintenance pathway facilitating the replication and distribution of mtDNA within cells. MPV17 has functional associations with other proteins like POLG which is the catalytic subunit of the mitochondrial DNA polymerase responsible for replication and repair. This relationship ensures the stability and transcription of mitochondrial genome necessary for proper cellular energy management.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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