Human MPZL1 (MPZL) knockout HeLa cell lysate
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MPZL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
MPZL1b, Myelin protein zero like protein 1, OTTHUMP00000032519, PZR, PZR1b, PZRa, PZRb, immunoglobulin family transmembrane protein, myelin protein zero like 1, myelin protein zero like protein 1 precursor, myelin protein zero like protein MPZL1b, protein zero related
- WB
Lab
Western blot - Human MPZL1 (MPZL) knockout HeLa cell lysate (AB258053)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : MPZL1 knockout HeLa cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : 293T cell lysate (20 ug)
ab151541 was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265332 (knockout cell lysate ab258053) was used. Wild-type and MPZL knockout samples were subjected to SDS-PAGE. ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MPZL antibody [EPR9229] (<a href='/en-us/products/primary-antibodies/mpzl-antibody-epr9229-ab151541'>ab151541</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MPZL1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human MPZL1 (MPZL) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mpzl1-mpzl-knockout-hela-cell-line-ab265332'>ab265332</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
293T cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MPZL1 (MPZL) knockout HeLa cell lysate (AB258053)
Homozygous : 1 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MPZL1 supports critical functions like cell proliferation and differentiation through its interactions with other cellular components. It often associates with cytoplasmic signaling proteins suggesting its role in forming larger signaling complexes. MPZL1 engages in downstream signaling pathways when it binds to ligands influencing cellular dynamics and enabling proper tissue development and maintenance. Its interaction with other proteins furthers understanding of its involvement in cellular structural integrity.
Pathways
MPZL1 integrates into important cellular signaling cascades including the MAPK and PI3K-AKT pathways. Within these pathways MPZL1 modulates activity by contacting proteins like GRB2 and PIK3R1 influencing signals that are important for cellular growth and survival. Its activity in these pathways suggests an adaptive role in regulating cellular responses to external stimuli contributing to proper physiological function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB248994
Anti-MPZL antibody [EPR9229] - BSA and Azide free
RabMAb|Recombinant|KO Validated
![Western blot - Anti-MPZL antibody [EPR9229] - BSA and Azide free (AB248994)](https://content.abcam.com/products/images/mpzl-antibody-epr9229-bsa-and-azide-free-ab248994--western-blot-img3516.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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