Human MRAS knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
MRAS KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
View Alternative Names
FLJ42964, M-ras, Muscle RAS oncogene homolog, Muscle Ras oncogene homologue, Muscle Ras viral oncogene homolog, Muscle and microspikes Ras, R-ras3, RASM_HUMAN, Ras-related protein M-Ras, Ras-related protein R-Ras3, Related Ras viral oncogene homolog 3, XRas
- WB
Lab
Western blot - Human MRAS knockout HeLa cell lysate (AB257541)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : MRAS knockout HeLa cell lysate (20 ug)
Lane 3 : Human brain tissue lysate (20 ug)
ab176570 was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265510 (knockout cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MRas antibody [EPR12457] (<a href='/en-us/products/primary-antibodies/mras-antibody-epr12457-ab176570'>ab176570</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MRAS knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human MRAS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mras-knockout-hela-cell-line-ab265510'>ab265510</a>)
Lane 3:
Human brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MRAS knockout HeLa cell lysate (AB257541)
Allele-1 : 2 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human MRAS knockout HeLa cell lysate (AB257541)
Allele-2 : 1 bp insertion in exon3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.
Pathways
MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-MRas antibody [EPR12457] (AB176570)](https://content.abcam.com/products/images/mras-antibody-epr12457-ab176570--western-blot-img5924.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com