Human MRFAP1 knockout HEK-293T cell lysate
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MRFAP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
View Alternative Names
MOFA1_HUMAN, MORF4 family-associated protein 1, Mof4 family associated protein 1, Mrfap1, OTTHUMP00000155159, OTTHUMP00000217763, PAM14, PGR1, Protein PGR1, Protein associated with MRG 14 kDa, Protein associated with MRG of 14 kDa, T cell activation protein
- Sanger seq
Unknown
Sanger Sequencing - Human MRFAP1 knockout HEK-293T cell lysate (AB263256)
Homozygous : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein MRFAP1 contributes to the regulation of transcription by serving as a scaffold linking histone acetylation and methylation. This action fosters interactions within the MRN complex which includes MRG15. Through these connections MRFAP1 influences chromatin structure and as a result gene expression. Its role is significant in regulating cellular senescence and differentiation.
Pathways
MRFAP1 is actively involved in the chromatin modification pathway. This pathway coordinates with the action of other proteins such as MRG15 to alter histone acetylation levels impacting transcriptional regulation. Additionally MRFAP1 engages in the DNA damage repair pathway assisting another protein known as RNF8. These interactions anchor MRFAP1 in pivotal cellular functions relating to genetic stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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