Human MRPL2 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
MRPL2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 100 bp deletion in exon1 and 43 bp insertion in exon1 and 7 bp deletion in exon1.
View Alternative Names
39S ribosomal protein L2, 39S ribosomal protein L2, mitochondrial precursor, CGI22, L2mt, Mitochondrial ribosomal protein L2, RM02_HUMAN, RPML14, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell lysate (AB263257)
Allele-1 : 100 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell lysate (AB263257)
Allele-2 : 7 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL2 knockout HEK-293T cell lysate (AB263257)
Allele-3 : 43 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRPL2 contributes to efficient protein manufacturing within the mitochondria by being a part of the mitochondrial ribosomal complex. This complex is responsible for translating 13 essential polypeptides that integrate into the inner mitochondrial membrane and are important for oxidative phosphorylation. MRPL2's presence ensures the translation process occurs smoothly directly influencing cellular energy production. The integrity of mitochondrial protein synthesis significantly affects overall cellular metabolism and energy balance.
Pathways
MRPL2 influences mitochondrial translation and oxidative phosphorylation. It works within pathways that maintain cellular energy production directly impacting cell survival and function. During these processes MRPL2 interacts with essential components like mitochondrial ATP synthase and cytochrome c oxidase facilitating energy conversion from food into usable ATP. These close interactions demonstrate MRPL2's importance in sustaining energy homeostasis and cellular respiration.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com