Human MRPL40 knockout HeLa cell lysate
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MRPL40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
39S ribosomal protein L40, 39S ribosomal protein L40 mitochondrial, 39S ribosomal protein L40, mitochondrial precursor, FLJ41774, L40mt, MGC9400, MRP 40, MRP L22, MRP-L40, Mitochondrial ribosomal protein L40, NLVCF, Nuclear localization signal deleted in velocardiofacial syndrome, Nuclear localization signal-containing protein deleted in velocardiofacial syndrome, RM40_HUMAN, URIM, Up-regulated in metastasis, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL40 knockout HeLa cell lysate (AB258971)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MRPL40 knockout HeLa cell lysate (AB258971)
Allele-1 : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRPL40 contributes significantly to mitochondrial ribosome assembly and function forming part of the 39S large subunit. The protein's involvement in the mitochondrial ribosomal complex is critical for efficient mitochondrial protein synthesis. This complex ensures the proper production of proteins required for the oxidative phosphorylation system thereby influencing cellular energy metabolism.
Pathways
MRPL40 functions are integral to the mitochondrial translation pathway. This pathway plays a role in energy production and cellular respiration. MRPL40 interacts with other mitochondrial ribosomal proteins such as MRPS12 forming a coordinated system ensuring effective mitochondrial translation. Its proper function impacts oxidative phosphorylation and cellular metabolism pathways influencing energy generation in the cell.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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