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AB263258

Human MRPL50 knockout HEK-293T cell lysate

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MRPL50 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.
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Sanger Sequencing - Human MRPL50 knockout HEK-293T cell lysate (AB263258)
  • Sanger seq

Unknown

Sanger Sequencing - Human MRPL50 knockout HEK-293T cell lysate (AB263258)

Homozygous : 7 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

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Complementary Products

Cell Lines & Lysates

AB266832

Human MRPL50 knockout HEK-293T cell line

Sanger Sequencing - Human MRPL50 knockout HEK-293T cell line (AB266832)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MRPL50
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL50 also known as Mitochondrial Ribosomal Protein L50 is a component of the mitochondrial ribosome. With a molecular mass of approximately 16.5 kDa MRPL50 is located within the mitochondria the energy-producing centers of the cell. It forms part of the large 39S subunit of mitochondrial ribosomes and is essential for protein synthesis within mitochondria. Expression of MRPL50 occurs in tissues with high energy demands like muscle and brain reflecting its role in mitochondrial function.
Biological function summary

MRPL50 plays an essential role in the formation and function of the mitochondrial ribosomal large subunit. A component of the mitochondrial ribosomal complex it contributes to translating mitochondrial mRNA into proteins necessary for oxidative phosphorylation. This function is critical for the maintenance of mitochondrial activity and by extension cellular energy metabolism. The interactions within the ribosomal complex help ensure the efficient synthesis of proteins encoded by mitochondrial DNA.

Pathways

The function of MRPL50 integrates into the oxidative phosphorylation and mitochondrial translation pathways. This protein is important for the synthesis of components in the electron transport chain a pivotal pathway in energy production. MRPL50 operates together with other mitochondrial ribosomal proteins in translating mitochondrial genes into functional proteins which link into pathways like those involving the NADH dehydrogenase complex.

Malfunctions or mutations in MRPL50 associate with mitochondrial diseases such as mitochondrial myopathy and neurodegenerative disorders. The role of MRPL50 in producing mitochondrial proteins links it to conditions where mitochondrial energy production is compromised. Related proteins in these diseases include those involved in the assembly and stability of the mitochondrial ribosomes as well as key constituents of the oxidative phosphorylation system.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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