MRPS28 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Alternative names
28S ribosomal protein S28, 28S ribosomal protein S35, HSPC007, Mitochondrial 28S ribosomal protein S35, Mitochondrial ribosomal protein S28, RT28_HUMAN, S28mt, S35mt, mitochondrial
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MRPS28 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
- Concentration
Properties
- Gene name
- MRPS28
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MRPS28 also known as Mitochondrial Ribosomal Protein S28 is a protein that plays an important role in mitochondrial protein synthesis. It is a component of the small subunit of the mitochondrial ribosome which is important for the translation of mitochondrial mRNAs. This protein has a molecular mass of approximately 12 kDa and is mainly expressed in the mitochondria an organelle present in most eukaryotic cells. As a mitochondrial ribosomal protein MRPS28 is part of the machinery responsible for producing proteins that are essential for mitochondrial function and energy production.
- Biological function summary
MRPS28 is involved in the assembly and stability of the mitochondrial ribosomal small subunit complex. It ensures the proper translation of 13 mitochondrial-encoded polypeptides which are components of the oxidative phosphorylation system. MRPS28 interacts with other mitochondrial ribosomal proteins to form a functional ribosome supporting mitochondrial gene expression and cellular energy metabolism. The presence of MRPS28 in this complex is necessary for the maintenance of mitochondrial translation fidelity and efficiency.
- Pathways
MRPS28 plays a role in mitochondrial translation and oxidative phosphorylation pathways. It contributes to the synthesis of proteins that are integral to the electron transport chain an important part of energy production in cells. Proteins such as MRPS16 and MRPS18 are also involved within these same pathways working closely with MRPS28 to ensure efficient mitochondrial function. These pathways are essential to provide energy in the form of ATP supporting various cellular processes.
- Associated diseases and disorders
MRPS28 has connections to conditions associated with mitochondrial dysfunction such as mitochondrial myopathy and Leigh syndrome. Research has suggested that mutations affecting MRPS28 could impair mitochondrial translation leading to decreased energy production and resulting in these disorders. Through its role in these diseases MRPS28 is linked with other mitochondrial proteins like MRPL3 which when dysfunctional can also contribute to similar mitochondrial pathologies. Understanding the function and disease associations of MRPS28 may provide insights into potential therapeutic strategies for mitochondrial diseases.
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5 product images
Western blot - Human MRPS28 knockout HEK-293T cell lysate (ab263259)
Lane 1: Wild-type Raji cell lysate 20 μg
Lane 2: MS4A1 knockout Raji cell lysate 20 μg
Lane 3: A549 cell lysate 20 μg
Lane 4: HeLa cell lysate 20 μg
False colour image of Western blot: Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) ab279300 was shown to bind specifically to CD20. A band was observed at 33 kDa in wild-type Raji cell lysates with no signal observed at this size in MS4A1 knockout cell line Human MS4A1 (CD20) knockout Raji cell line ab273871 (knockout cell lysate ab263259). To generate this image, wild-type and MS4A1 knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] (Anti-CD20 antibody [EP459Y] ab78237) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) (Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) ab279300) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: MS4A1 knockout Raji cell lysate at 20 µg
Lane 2: Western blot - Human MS4A1 (CD20) knockout Raji cell line (Human MS4A1 (CD20) knockout Raji cell line ab273871)
Lane 3: A549 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa, 33 kDa
Observed band size: 33 kDa
Western blot - Human MRPS28 knockout HEK-293T cell lysate (ab263259)
Lane 1: Wild-type Raji cell lysate 20 μg
Lane 2: MS4A1 knockout Raji cell lysate 20 μg
Lane 3: A549 cell lysate 20 μg
Lane 4: HeLa cell lysate 20 μgFalse colour image of Western blot: Anti-CD20 antibody [EP459Y] – Mouse IgG2a (Chimeric) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD20 antibody [EP459Y] - Mouse IgG2a (Chimeric) ab279299 was shown to bind specifically to CD20. A band was observed at 33 kDa in wild-type Raji cell lysates with no signal observed at this size in MS4A1 knockout cell line Human MS4A1 (CD20) knockout Raji cell line ab273871 (knockout cell lysate ab263259). To generate this image, wild-type and MS4A1 knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] (Anti-CD20 antibody [EP459Y] ab78237) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] - Mouse IgG2a (Chimeric) (Anti-CD20 antibody [EP459Y] - Mouse IgG2a (Chimeric) ab279299) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: MS4A1 knockout Raji cell lysate at 20 µg
Lane 2: Western blot - Human MS4A1 (CD20) knockout Raji cell line (Human MS4A1 (CD20) knockout Raji cell line ab273871)
Lane 3: A549 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa, 33 kDa
Observed band size: 33 kDa
Western blot - Human MRPS28 knockout HEK-293T cell lysate (ab263259)
Lane 1: Wild-type Raji cell lysate 20 μg
Lane 2: MS4A1 knockout Raji cell lysate 20 μg
Lane 3: A549 cell lysate 20 μg
Lane 4: HeLa cell lysate 20 μgFalse colour image of Western blot: Anti-CD20 antibody [EP459Y] - Mouse IgG1 (Chimeric) staining, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD20 antibody [EP459Y] - Mouse IgG1 (Chimeric) ab279298 was shown to bind specifically to CD20. A band was observed at 33 kDa in wild-type Raji cell lysates with no signal observed at this size in MS4A1 knockout cell line Human MS4A1 (CD20) knockout Raji cell line ab273871 (knockout cell lysate ab263259). To generate this image, wild-type and MS4A1 knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] - Mouse IgG1 (Chimeric) (Anti-CD20 antibody [EP459Y] - Mouse IgG1 (Chimeric) ab279298) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-CD20 antibody [EP459Y] (Anti-CD20 antibody [EP459Y] ab78237) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: MS4A1 knockout Raji cell lysate at 20 µg
Lane 2: Western blot - Human MS4A1 (CD20) knockout Raji cell line (Human MS4A1 (CD20) knockout Raji cell line ab273871)
Lane 3: A549 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa, 33 kDa
Observed band size: 33 kDa
Sanger Sequencing - Human MRPS28 knockout HEK-293T cell lysate (ab263259)
Allele-2: Insertion of the selection cassette in exon1
Sanger Sequencing - Human MRPS28 knockout HEK-293T cell lysate (ab263259)
Allele-1: 1 bp insertion in exon1
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