Human MRPS7 knockout HeLa cell lysate
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MRPS7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1 and 2 bp deletion in exon1 and 31 bp deletion in exon1.
View Alternative Names
28S ribosomal protein S7, 28S ribosomal protein S7, mitochondrial precursor, 30S ribosomal protein S7 homolog, MRP S, Mitochondrial ribosomal protein S7, RPMS7, RT07_HUMAN, S7mt, bMRP-27a, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human MRPS7 knockout HeLa cell lysate (AB258972)
Allele-1 : 31 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MRPS7 knockout HeLa cell lysate (AB258972)
Allele-2 : 11 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MRPS7 knockout HeLa cell lysate (AB258972)
Allele-3 : 2 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MRPS7 is involved in mitochondrial translation a process critical for cellular respiration and energy production. This protein is part of the mitochondrial ribosomal complex which includes both proteins and rRNA. By playing an active role in assembling and stabilizing the ribosome MRPS7 helps in translating mRNA transcribed from mitochondrial DNA. This translation is necessary for the production of essential components of the electron transport chain which generates ATP.
Pathways
MRPS7 engages in the mitochondrial translation pathway which ensures the synthesis of proteins required for the oxidative phosphorylation pathway. It interacts with other mitochondrial ribosomal proteins like MRPS28 to form a functional ribosome. Through these interactions MRPS7 helps maintain the efficiency and fidelity of mitochondrial protein synthesis directly affecting cellular energy metabolism and homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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