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AB263260

Human MSH5 knockout HeLa cell lysate

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MSH5 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 40 bp deletion in exon6.
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Sanger Sequencing - Human MSH5 knockout HeLa cell lysate (AB263260)
  • Sanger seq

Unknown

Sanger Sequencing - Human MSH5 knockout HeLa cell lysate (AB263260)

Homozygous : 40 bp deletion in exon6

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 40 bp deletion in exon6.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265170

Human MSH5 knockout HeLa cell line

Sanger Sequencing - Human MSH5 knockout HeLa cell line (AB265170)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MSH5
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MutS Homolog 5 (MSH5) also known as human homolog of bacterial MutS plays a role in DNA repair and meiotic recombination. It is a protein composed of 834 amino acids with a molecular mass of approximately 93.7 kDa. MSH5 shows significant expression in reproductive tissues such as testes and ovaries indicating its importance in germ cell development. Other tissues like kidney and lung also express MSH5 suggesting roles beyond reproduction.
Biological function summary

MSH5 participates in maintaining genomic stability especially during meiosis. It forms a heterodimer with MSH4 creating a functional complex necessary for efficient crossing-over and recombination events. This complex recognizes and repairs mismatches and insertion-deletion loops ensuring genetic diversity during gamete formation. MSH5 also interacts with proteins involved in the DNA damage response linking its function to broader DNA repair mechanisms.

Pathways

MSH5 plays important roles in the meiotic recombination checkpoint and mismatch repair (MMR) pathways. In the meiotic recombination pathway MSH5 works alongside proteins like MLH1 and MLH3 ensuring successful homologous recombination. During the mismatch repair pathway the MSH4-MSH5 complex recognizes and binds to DNA mismatches recruiting other repair proteins to resolve these issues preventing mutations that can affect genomic integrity.

Mutations or altered expression of MSH5 have associations with infertility and certain cancers such as testicular cancer. The MSH5 alterations might disrupt the repair of DNA mismatches or meiotic recombination leading to genetic instability. In cancer the MSH5 protein might exhibit abnormal interactions with other repair proteins like BRCA1 compromising DNA repair processes and facilitating cancer progression. Understanding MSH5's role could advance our knowledge on genetic disorders related to recombination failures and guide potential therapeutic strategies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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