Human MSL1 knockout HeLa cell lysate
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MSL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 1 bp insertion in exon 3.
View Alternative Names
MSL1-like 1, MSL1_HUMAN, Male-specific lethal 1 homolog, Male-specific lethal 1-like 1, Male-specific lethal-1 homolog 1, hMSL1, male specific lethal 1
- Sanger seq
Unknown
Sanger Sequencing - Human MSL1 knockout HeLa cell lysate (AB258973)
Allele-2 : 1 bp insertion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human MSL1 knockout HeLa cell lysate (AB258973)
Allele-1 : 13 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MSL1 participates in the assembly of the MSL complex. This multi-protein complex facilitates histone H4 lysine 16 acetylation which activates transcription. MSL1 specifically assists in recruiting the acetyltransferase MOF to target sites on the X chromosome. The expression level and activity of MSL1 are tightly regulated to ensure proper chromosomal balance in the organism. Its activity is not limited to dosage compensation but also influences other chromatin remodeling processes.
Pathways
MSL1 plays significant roles in chromatin modification pathways participating in transcriptional regulation actively. The protein aligns closely with MOF in these pathways forming a duo that heavily influences acetylation across chromatin landscapes. In addition to histone modification MSL1 connections extend to gene expression pathways responding to various cellular stimuli interfacing with proteins like MSL2 and MSL3 to regulate gene activation machinery.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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