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AB257542

Human MSN (Moesin) knockout HeLa cell lysate

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MSN KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
8 Images
Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Lab

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : MSN knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-Moesin antibody [EPR2428(2)] staining at 2 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151542 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Moesin antibody [EPR2428(2)] (<a href='/en-us/products/primary-antibodies/moesin-antibody-epr24282-ab151542'>ab151542</a>) at 2 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Collaborator

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : Human MSN knockout HeLa cell lysate (20 ug)

ab151542 was shown to react with MSN in wild-type HeLa cells in Western blot with loss of signal observed in MSN knockout cell line ab265020 (MSN knockout cell lysate ab257542). Wild-type HeLa and MSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab151542 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Moesin antibody [EPR2428(2)] (<a href='/en-us/products/primary-antibodies/moesin-antibody-epr24282-ab151542'>ab151542</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

false

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Collaborator

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : Human MSN knockout HeLa cell lysate (20 ug)

ab169789 was shown to react with MSN in wild-type HeLa cells in Western blot with loss of signal observed in MSN knockout cell line ab265020 (MSN knockout cell lysate ab257542). Wild-type HeLa and MSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab169789 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Moesin antibody [EPR2429(2)] (<a href='/en-us/products/primary-antibodies/moesin-antibody-epr24292-ab169789'>ab169789</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

false

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Lab

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : MSN knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-Moesin antibody [EPR2429(2)] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab169789 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Moesin antibody [EPR2429(2)] (<a href='/en-us/products/primary-antibodies/moesin-antibody-epr24292-ab169789'>ab169789</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Lab

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : MSN knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-Moesin antibody [MSN/492] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab216033 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Moesin antibody [MSN/492] (<a href='/en-us/products/primary-antibodies/moesin-antibody-msn-492-ab216033'>ab216033</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Lab

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : MSN knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-Moesin antibody [EP1863Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52490 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Moesin antibody [EP1863Y] (<a href='/en-us/products/primary-antibodies/moesin-antibody-ep1863y-ab52490'>ab52490</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MSN knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-msn-moesin-knockout-hela-cell-line-ab265020'>ab265020</a>)

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Sanger Sequencing - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • Sanger seq

Unknown

Sanger Sequencing - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Homozygous : 1 bp insertion in exon 5

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)
  • WB

Collaborator

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (AB257542)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : Human MSN knockout HeLa cell lysate (20 ug)

ab52490 was shown to react with MSN in wild-type HeLa cells in Western blot with loss of signal observed in MSN knockout cell line ab265020 (MSN knockout cell lysate ab257542). Wild-type HeLa and MSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab52490 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Moesin antibody [EP1863Y] (<a href='/en-us/products/primary-antibodies/moesin-antibody-ep1863y-ab52490'>ab52490</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MSN (Moesin) knockout HeLa cell lysate (ab257542)

Predicted band size: 68 kDa

false

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB52490

Anti-Moesin antibody [EP1863Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Moesin antibody [EP1863Y] (AB52490)

  • 5
  • 5
Proteins & Peptides

AB64309

Recombinant Human Moesin protein

SDS-PAGE - Recombinant Human Moesin protein (AB64309)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257542-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human MSN knockout HeLa cell lysate", "number":"AB257542-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257542-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
MSN
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Moesin also known as MSN or moesin protein is part of the ERM (ezrin radixin moesin) family which links the actin cytoskeleton to the plasma membrane. It weighs around 68 kDa. This protein is widely expressed in various tissues notably in endothelial cells and other cells with high cytoskeletal dynamics. Moesin plays an essential role in maintaining cell shape polarity and enabling cellular processes like migration and adhesion.
Biological function summary

This protein serves as a crosslinker between the plasma membrane and the actin cytoskeleton influencing signal transduction pathways. Moesin participates significantly in cellular processes like cytokinesis and microvilli formation by binding actin filaments. It is part of several functional complexes ensuring proper cytoskeletal organization and cellular dynamics. Moesin's interactions with other proteins like Rho GTPases regulate its functions aiding cellular morphology and polarity.

Pathways

Moesin is actively involved in the RhoA-ROCK pathway. This pathway is fundamental to actin cytoskeletal reorganization and cell contraction. Moesin interacts with proteins such as radixin and ezrin ensuring cohesion in cytoskeletal rearrangements. Through these interactions Moesin contributes to cell motility and various signaling cascades necessary for cellular responses.

Moesin is associated with conditions such as cancer and immune system disorders. Aberrant expression of moesin can contribute to enhanced cellular migration and invasion which are mechanisms seen in cancer metastasis. Moesin's interaction with proteins like ezrin in cancer progression has been documented. Also in immune disorders altered moesin activity can affect leukocyte transmigration disrupting efficient immune responses.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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