Human MSX1 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human MSX1 knockout A549 cell lysate (AB258526)
Homozygous : 1 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MSX1 modulates a variety of developmental processes including craniofacial morphogenesis and limb formation. It operates as part of a DNA-binding complex that influences the transcription of target genes essential for proper tissue differentiation and formation. Through its activity MSX1 affects cell proliferation and apoptosis ensuring the accurate development of specific structures in the organism.
Pathways
MSX1 interacts with the WNT signaling and bone morphogenetic protein (BMP) pathways. In the WNT signaling pathway MSX1 modulates the expression of genes involved in the regulation of developmental processes and cell fate determination. It also interacts with BMP-related proteins playing an important role in skeletal formation and patterning demonstrating connections with other proteins like BMP4 and LEF1.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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