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AB263261

Human MT1X knockout HeLa cell lysate

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MT1X KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
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Sanger Sequencing - Human MT1X knockout HeLa cell lysate (AB263261)
  • Sanger seq

Unknown

Sanger Sequencing - Human MT1X knockout HeLa cell lysate (AB263261)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265309

Human MT1X knockout HeLa cell line

Sanger Sequencing - Human MT1X knockout HeLa cell line (AB265309)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MT1X
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT1X also known as metallothionein-1X is a protein with a molecular mass of approximately 6-7 kDa. This protein belongs to the metallothionein family and plays an essential role in metal ion binding. MT1X primarily expresses in the liver kidneys and pancreas where it helps in stabilizing metal ions and detoxifying heavy metals. The structure of MT1X allows it to bind with metals like zinc and cadmium assisting in maintaining metal homeostasis.
Biological function summary

Metallothionein-1X acts as a protective agent against oxidative damage by binding and sequestering metal ions therefore preventing the generation of harmful reactive oxygen species. It does not form part of a complex independently but interacts with other proteins like metallothionein-2A to enhance cellular antioxidant defense. MT1X regulates metal ion concentrations within cells and contributes to the detoxification process safeguarding cells against metal-induced cytotoxicity.

Pathways

MT1X functions within pathways related to metal ion metabolism and detoxification processes. In the context of metal ion metabolism it interacts closely with proteins such as zinc transporter proteins maintaining optimal zinc levels in cells. MT1X also associates with the oxidative stress response pathway where it collaborates with proteins like glutathione to mitigate damage from oxidative stress.

Alterations in MT1X expression link to disorders like cancer and neurodegenerative diseases. In cancer an altered MT1X expression can impact cellular redox state and metal ion balance contributing to tumor progression. Its potential interplay with proteins like metallothionein-2A may influence cellular responses to oxidative stress in neurodegenerative diseases affecting neuronal survival and function. Understanding MT1X relationships with these proteins might offer insights into the pathogenesis and therapeutic strategies for these disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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