Human MTHFD1L knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell lysate (AB258976)
Allele-1 : 8 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell lysate (AB258976)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell lysate (AB258976)
Allele-2 : 1 bp insertion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MTHFD1L catalyzes reactions converting methylene-THF to methenyl-THF and then to formyl-THF utilizing NADP+ as a cofactor. It does not function as a standalone component; instead it may operate as part of a larger complex engaged in one-carbon metabolism. Its activity contributes to the synthesis of important biomolecules such as nucleotides and amino acids emphasizing its role in DNA synthesis and repair.
Pathways
The folate-mediated one-carbon metabolism uses MTHFD1L to manage one-carbon units. This enzyme's function fits into the folate pathway important for nucleotide biosynthesis. It connects to other proteins such as MTHFD1 and SHMT1 which also partake in folate metabolism supporting interconnected biochemical pathways in various cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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