Human MTHFD2 knockout HEK-293T cell lysate
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MTHFD2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 5 bp deletion in exon1.
View Alternative Names
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, Descriptions, MGC82516, MTDC_HUMAN, Methenyltetrahydrofolate cyclohydrolase, NAD-dependent methylene tetrahydrofolate dehydrogenase, NMDMC, methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methylenetetrahydrofolate dehydrogenase 2, mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent)
- WB
Unknown
Western blot - Human MTHFD2 knockout HEK-293T cell lysate (AB258528)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : MTHFD2 knockout HEK293T cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)
ab56772 was shown to specifically react with MTHFD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266383 (knockout cell lysate ab258528) was used. Wild-type and MTHFD2 knockout samples were subjected to SDS-PAGE. ab56772 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD2 knockout HEK-293T cell lysate (AB258528)
Allele-1 : 5 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD2 knockout HEK-293T cell lysate (AB258528)
Allele-3 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MTHFD2 knockout HEK-293T cell lysate (AB258528)
Allele-2 : 1 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.
Pathways
MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB307428
Anti-MTHFD2 antibody [EPR26938-20]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)](https://content.abcam.com/products/images/mthfd2-antibody-epr26938-20-ab307428--flow-cytometry-intracellular-img405590.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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