Human MTMR2 knockout HeLa cell lysate
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MTMR2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 5 and Insertion of the selection cassette in exon 5.
View Alternative Names
CMT4B, CMT4B1, KIAA1073, MTMR2_HUMAN, Myotubularin-related protein 2, OTTHUMP00000204445, OTTHUMP00000204446, OTTHUMP00000204447, OTTHUMP00000204448, Phosphatidylinositol 3 phosphate phosphatase, Phosphatidylinositol 3,5 bisphosphate 3 phosphatase
- Sanger seq
Unknown
Sanger Sequencing - Human MTMR2 knockout HeLa cell lysate (AB263262)
Allele-2 : Insertion of the selection cassette in exon 5
- Sanger seq
Unknown
Sanger Sequencing - Human MTMR2 knockout HeLa cell lysate (AB263262)
Allele-1 : 2 bp deletion in exon 5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MTMR2 facilitates the regulation of membrane trafficking and endosomal processing by controlling phosphoinositide levels. It operates as part of larger protein complexes interacting with other members of the myotubularin family. This collaboration is important for maintaining cellular dynamics and integrity influencing processes such as cell growth and cytoskeletal organization. These actions are necessary for proper nerve function and development.
Pathways
MTMR2 is mainly involved in phosphatidylinositol signaling and endocytic pathways. This protein interacts closely with PI3K influencing the PI3K-Akt signaling pathway critical for cell survival and proliferation. Connections also occur with proteins such as MTMR5 which facilitate further regulatory controls. These pathways highlight the role of MTMR2 in cellular signal transduction and its influence on cell morphology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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