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AB263267

Human MVB12A (FAM125A) knockout HeLa cell lysate

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MVB12A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 40 bp deletion in exon1.
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Sanger Sequencing - Human MVB12A (FAM125A) knockout HeLa cell lysate (AB263267)
  • Sanger seq

Unknown

Sanger Sequencing - Human MVB12A (FAM125A) knockout HeLa cell lysate (AB263267)

Allele-1 : 1 bp insertion in exon1

Sanger Sequencing - Human MVB12A (FAM125A) knockout HeLa cell lysate (AB263267)
  • Sanger seq

Unknown

Sanger Sequencing - Human MVB12A (FAM125A) knockout HeLa cell lysate (AB263267)

Allele-2 : 40 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 40 bp deletion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265886

Human MVB12A (FAM125A) knockout HeLa cell line

Sanger Sequencing - Human MVB12A (FAM125A) knockout HeLa cell line (AB265886)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MVB12A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM125A also known as MVB12A is a protein involved in the endosomal sorting complex required for transport particularly the ESCRT-I complex. It is about 26 kDa in mass and plays a role in cargo recognition during the sorting process. FAM125A is expressed in many tissues including the central nervous system indicating its wide functional relevance.
Biological function summary

FAM125A participates in the ESCRT machinery which is essential for the regulation of endosomal trafficking. It associates with other proteins such as TSG101 within the ESCRT-I complex facilitating the sorting of ubiquitinated membrane proteins into multivesicular bodies. This activity is vital for processes like receptor down-regulation and virus budding indicating its significant involvement in cellular homeostasis.

Pathways

FAM125A significantly engages in the endocytic and lysosomal degradation pathways. It works closely with ESCRT-I complex partners such as VPS37 and TSG101 aiding in membrane remodeling and protein trafficking. The ESCRT pathway is important for maintaining proper cell signaling and membrane composition linking FAM125A functionally to cellular maintenance and response processes.

FAM125A has associations with neurogenerative conditions and certain cancers. Disrupted endosomal sorting might contribute to pathological states where protein aggregation or receptor recycling is impaired such as in Alzheimer's disease. Additionally FAM125A and ESCRT-related proteins like VPS37 may play roles in tumor progression and metastasis as proper endosome function is necessary to control cell division and migration signals.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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