JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263850

Human MYC (c-Myc) knockout HEK-293T cell lysate

5

(1 Review)

|

(0 Publication)

MYC KO cell lysate available now. KO validated by Immunohistochemistry, Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous (4N): 1bp T insertion (2N); 8 bp deletion and C to T insertion (1N); 4 bp deletion in exon 2 (1N).

View Alternative Names

AU016757, Avian myelocytomatosis viral oncogene homolog, Cellular myelocytomatosis oncogene, Class E basic helix-loop-helix protein 39, MGC105490, MRTL, MYCC, MYC_HUMAN, Myc protein, Myc proto-oncogene protein, Myc2, Myelocytomatosis oncogene, Myelocytomatosis oncogene a, Niard, Nird, OTTHUMP00000158589, OTTHUMP00000227763, Oncogene Myc, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, myc-related translation/localization regulatory factor, myca, oncogene c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian), zc-myc

6 Images
Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYC knockout HEK-293T cell lysate 20 μg
Lane 3 : Jurkat cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg
False colour image of Western blot : Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC knockout cell line ab256500 (knockout cell lysate ab263850). The band observed in the knockout lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC knockout HEK-293T cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-9e10-ab32'>ab32</a>) at 1/200 dilution

Lane 2:

MYC knockout HEK-293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa

false

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate 20 μg
Lane 3 : Jurkat cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg
False colour image of Western blot : Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

Lanes 1 - 4:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-9e10-ab32'>ab32</a>) at 1/200 dilution

Lanes 1 - 4:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-9e10-ab206486'>ab206486</a>) at 1/200 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MYC CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-myc-c-myc-knockout-hek-293t-cell-line-ab256500'>ab256500</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa,49 kDa

false

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate 20 μg
Lane 3 : Jurkat cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg
False colour image of Western blot : Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Myc tag antibody [Myc.A7] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-myca7-ab18185'>ab18185</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MYC CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-myc-c-myc-knockout-hek-293t-cell-line-ab256500'>ab256500</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa,49 kDa

false

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYC knockout HEK-293T cell lysate 20 μg
Lane 3 : Jurkat cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg
False colour image of Western blot : Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC knockout cell line ab256500 (knockout cell lysate ab263850). The band observed in the knockout lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC knockout HEK-293T cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Myc tag antibody [Myc.A7] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-myca7-ab18185'>ab18185</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MYC knockout HEK-293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa

false

Sanger Sequencing - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • Sanger seq

Unknown

Sanger Sequencing - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Homozygous : 1 bp insertion in exon 2

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (AB263850)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate 20 μg
Lane 3 : Jurkat cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg
False colour image of Western blot : Anti-c-Myc antibody [Y69] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32072 was shown to bind specifically to c-Myc. A band was observed at 45/57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 45/57 kDa is likely to represent a truncated form of c-Myc. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-c-Myc antibody [Y69] - ChIP Grade (<a href='/en-us/products/primary-antibodies/c-myc-antibody-y69-chip-grade-ab32072'>ab32072</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (ab263850) at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 45 kDa,57 kDa

false

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Immunohistochemistry,Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous (4N): 1bp T insertion (2N); 8 bp deletion and C to T insertion (1N); 4 bp deletion in exon 2 (1N)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263850-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human MYC knockout HEK293T cell lysate", "number":"AB263850-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263850-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
MYC
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Immunohistochemistry, Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Cell Lines & Lysates

AB256500

Human MYC (c-Myc) knockout HEK-293T cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

  • 5
  • 1
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Proteins & Peptides

AB169901

Recombinant human c-Myc protein (Active)

  • 5
  • 3
Assay Kits

AB207200

c-Myc Transcription Factor Assay Kit (Colorimetric)

Functional Studies - c-Myc Transcription Factor Assay Kit (Colorimetric) (AB207200)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com