Human MYL6B (MLC1SA) knockout HEK-293T cell lysate
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MYL6B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 2.
View Alternative Names
MYL6B_HUMAN, Myosin alkali light chain 1 slow a, Myosin light chain 1 slow a, Myosin light chain 1 slow-twitch muscle A isoform, Myosin light chain 6B, Myosin light chain 6B alkali smooth muscle and non muscle, Myosin light polypeptide 6B, Myosin light polypeptide 6B alkali smooth muscle and non muscle, Smooth muscle and non muscle myosin alkali light chain 6B, Smooth muscle and nonmuscle myosin light chain alkali 6B
- Sanger seq
Unknown
Sanger Sequencing - Human MYL6B (MLC1SA) knockout HEK-293T cell lysate (AB263269)
Homozygous : 2 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MLC1SA contributes to muscle contraction regulation by modulating the interaction between actin and myosin. It is part of a complex that includes myosin heavy chains and other associated proteins. Through phosphorylation events MLC1SA adjusts the sensitivity of muscle fibers to calcium which is critical for the fine-tuning of muscle responses to stimuli. This subtle modulation is important for activities that rely on sustained muscle tension.
Pathways
Myosin light chain signaling involves MLC1SA as a central component in muscular contraction pathways. It plays a role in the myosin-actin crossbridge cycle aiding the efficient conversion of ATP into mechanical work. MLC1SA interacts with other proteins such as myosin heavy chains and calmodulin. These interactions are critical in pathways like the calcium signaling pathway and muscle contraction pathway where calcium ions serve as triggers for changes in muscle cell activity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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