Human MYOF (Myoferlin) knockout HeLa cell lysate
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MYOF KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 6.
View Alternative Names
FER1L3, Fer 1 like 3, myoferlin (C. elegans), Fer 1 like family member 3, Fer-1-like protein 3, MYOF_HUMAN, Myoferlin
- WB
Lab
Western blot - Human MYOF (Myoferlin) knockout HeLa cell lysate (AB257547)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : MYOF knockout HeLa cell lysate (20 ug)
Lane 3 : MDA-MB-231 cell lysate (20 ug)
Lane 4 : SK-BR-3 cell lysate (20 ug)
ab178386 was shown to specifically react with Myoferlin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265782 (knockout cell lysate ab257547) was used. Wild-type and Myoferlin knockout samples were subjected to SDS-PAGE. ab178386 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Myoferlin antibody [EPR18887] (<a href='/en-us/products/primary-antibodies/myoferlin-antibody-epr18887-ab178386'>ab178386</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MYOF knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human MYOF (Myoferlin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-myof-myoferlin-knockout-hela-cell-line-ab265782'>ab265782</a>)
Lane 3:
MDA-MB-231 cell lysate at 20 µg
Lane 4:
SK-BR-3 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 180 kDa,250 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MYOF (Myoferlin) knockout HeLa cell lysate (AB257547)
Homozygous : 10 bp deletion in exon 6
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein supports membrane dynamics and stabilization. Myoferlin is not part of a large complex but interacts with other proteins at the cellular membrane. It regulates cellular processes such as proliferation differentiation and endocytosis. In muscle cells it assists in the organization and function of the cytoskeleton maintaining proper cellular architecture and signaling.
Pathways
Myoferlin engages in critical roles in pathways related to cell membrane repair and trafficking. It associates with pathways like the myogenesis pathway and integrin signaling. In these pathways it interacts with other proteins such as dysferlin and caveolin-3 which facilitate membrane repair and receptor localization respectively. Myoferlin ensures efficient cellular response to membrane damage maintaining tissue integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)](https://content.abcam.com/products/images/myoferlin-antibody-epr18887-ab178386--western-blot-img5627.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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