Human MYSM1 knockout HEK-293T cell lysate
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MYSM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon8 and 2 bp insertion in exon8.
View Alternative Names
2A-DUB, Histone H2A deubiquitinase, Histone H2A deubiquitinase MYSM1, KIAA1915, MYSM1 Myb-like, SWIRM and MPN domains 1, MYSM1_HUMAN, Myb like, SWIRM and MPN domain containing protein, Myb-like, Myb-like, SWIRM and MPN domains 1, RP4-592A1.1, SWIRM and MPN domain-containing protein 1
- WB
Lab
Western blot - Human MYSM1 knockout HEK-293T cell lysate (AB257548)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : MYSM1 knockout HEK-293T cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : RT-4 cell lysate 20 μg
False colour image of Western blot : Anti-MYSM1 antibody [EPR18657] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab193081 was shown to bind specifically to MYSM1. A band was observed at 110 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYSM1 knockout cell line ab267297 (knockout cell lysate ab257548). To generate this image, wild-type and MYSM1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-MYSM1 antibody [EPR18657] (<a href='/en-us/products/primary-antibodies/mysm1-antibody-epr18657-ab193081'>ab193081</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
MYSM1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human MYSM1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mysm1-knockout-hek-293t-cell-line-ab267297'>ab267297</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
RT-4 cell lysate at 20 µg
Predicted band size: 95 kDa
Observed band size: 110 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MYSM1 knockout HEK-293T cell lysate (AB257548)
Allele-2 : 2 bp insertion in exon8
- Sanger seq
Unknown
Sanger Sequencing - Human MYSM1 knockout HEK-293T cell lysate (AB257548)
Allele-1 : 1 bp insertion in exon8
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MYSM1 plays an important role in regulating gene expression during processes like hematopoiesis and immune development. It forms part of a chromatin remodeling complex that modulates transcriptional activity by altering nucleosome configuration. This function is essential for proper stem cell maintenance and differentiation by ensuring that transcriptionally active and inactive regions are appropriately maintained and accessed.
Pathways
MYSM1 participates in the ubiquitin-proteasome pathway and is involved in DNA damage response. It associates closely with proteins like TP53 where it aids in governing cell cycle progression and apoptosis. MYSM1 also interacts with histone modification machinery exerting effects on transcriptional silencing and activation particularly influencing genes associated with immune cell lineage commitment.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-MYSM1 antibody [EPR18657] (AB193081)](https://content.abcam.com/products/images/mysm1-antibody-epr18657-ab193081--western-blot-img158464.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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