Human MZT1 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human MZT1 knockout HeLa cell lysate (AB263270)
Homozygous : 55 bp insertion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The small MZT1 protein integrates into the core structure of the mitotic apparatus by being part of the γ-tubulin ring complex (γ-TuRC). This complex is important for microtubule nucleation at the centrosome. By ensuring proper organization of the microtubules MZT1 supports accurate chromosomal segregation and cell division. These functions make MZT1 significant in maintaining genomic stability during cell replication.
Pathways
Ensuring precise spindle organization and function involves MZT1 in the regulation of the cell cycle and mitotic checkpoint pathways. It connects with proteins such as γ-tubulin and other spindle-associated proteins to facilitate microtubule dynamics. These interactions place MZT1 firmly within pathways that oversee correct mitotic progression and check for errors during cell division preventing chromosomal instability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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