Human MZT2B knockout HEK-293T cell lysate
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MZT2B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1.
View Alternative Names
MZT2B_HUMAN, Mitotic-spindle organizing protein 2B, Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2B
- Sanger seq
Unknown
Sanger Sequencing - Human MZT2B knockout HEK-293T cell lysate (AB263271)
Homozygous : 29 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The MZT2B protein contributes to the proper formation and function of the mitotic spindle. It is an integral part of the gamma-tubulin ring complex which is critical for microtubule nucleation at centrosomes. This complex ensures the stability and anchoring of microtubules facilitating accurate and equal chromosome segregation during mitosis. Proper spindle assembly is essential for optimal cell division and proliferation.
Pathways
The MZT2B protein plays a prominent role in the mitotic spindle assembly pathway and the cell cycle checkpoint pathway. It associates with proteins like gamma-tubulin and other components of the spindle assembly checkpoint machinery ensuring that all chromosomes are precisely aligned and attached to spindle fibers before proceeding to the next phase of cell division. The interplay between MZT2B and these pathways ensures successful mitosis and genomic stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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