Human NABP2 (hSSB1) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NABP2 (hSSB1) knockout HEK-293T cell lysate (AB258983)
Homozygous : 2 bp deletion in exon 3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a significant role in DNA damage response and repair. hSSB1 forms a complex with the INTS3 protein as part of the MRN (MRE11-RAD50-NBS1) complex to effectively respond to DNA double-strand breaks. This action is critical to initiate the appropriate signaling cascade that leads to DNA repair. The protein's expression and activity need tight regulation to ensure genome integrity and to prevent mutations.
Pathways
HSSB1 is involved in the homologous recombination repair pathway which is essential for error-free repair of DNA double-strand breaks. It interacts with BRCA1 and RAD51 which are key players in homologous recombination ensuring accurate DNA damage repair. Additionally hSSB1 participates in the ATR signaling pathway enhancing cell cycle checkpoint activation during replication stress where it collaborates with ATR and its substrates.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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