Human NADK knockout HEK-293T cell lysate
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NADK KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
FLJ13052, FLJ37724, NAD kinase, NADK_HUMAN, PPNK, Poly(P)/ATP NAD kinase, RP1-283E3.6, dJ283E3.1
- Sanger seq
Unknown
Sanger Sequencing - Human NADK knockout HEK-293T cell lysate (AB258985)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The conversion role played by NADK is essential for maintaining cellular redox status and for biosynthetic reactions that require NADPH such as fatty acid synthesis and detoxifying reactive oxygen species. NADK functions independently without being part of larger protein complexes. It facilitates key cellular processes by ensuring adequate levels of NADPH a necessary cofactor in various anabolic reactions and cellular defense mechanisms.
Pathways
NADK prominently features in the pentose phosphate pathway which is important for the production of NADPH. It interacts with enzymes like glucose-6-phosphate dehydrogenase (G6PD) which is also involved in producing NADPH through this pathway. Additionally NADK plays a role in the citric acid cycle where its activity supports energy metabolism by maintaining cellular NADP+/NADPH ratios.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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