Human NARF (IOP2) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell lysate (AB263272)
Allele-1 : 16 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell lysate (AB263272)
Allele-3 : 1 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell lysate (AB263272)
Allele-2 : 5 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IOP2 functions in intracellular signaling and membrane dynamics. It participates as a component of the ESCRT (endosomal sorting complexes required for transport) machinery. In this context IOP2 facilitates the sorting of ubiquitinated transmembrane proteins into the internal vesicles of multivesicular bodies. Its activity in this complex highlights the regulation of down-regulating surface receptors and contributing to the cellular homeostasis.
Pathways
IOP2 interacts with mechanisms controlling cellular growth and apoptosis. One of the significant pathways includes the mitogen-activated protein kinase (MAPK) signaling which influences cell proliferation. Within this pathway IOP2 relates to proteins such as ERK1/2 playing a role in the signal transduction processes. Additionally the protein contributes to the regulation of PI3K/AKT pathway impacting cell survival through its interactions within the signaling network.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com