NASP KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.
Alternative names
5033430J04Rik, AI131596, AI317140, D4Ertd767e, DKFZp547F162, Epcs32, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, MGC93869, NASP_HUMAN, Nuclear autoantigenic sperm protein, PRO1999, RP23 233B9.9, nuclear autoantigenic sperm protein (histone binding)
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NASP KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.
- Concentration
Properties
- Gene name
- NASP
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NASP also known as Nuclear Autoantigenic Sperm Protein functions as a histone chaperone. With a molecular mass of approximately 75 kDa NASP assists in the proper assembly and disassembly of nucleosome structures by binding to histones H1 and H2A-H2B. This protein mainly expresses in rapidly dividing tissues especially in the testes but also presents in the thymus and bone marrow. NASP maintains the supply of free histones aiding in DNA replication and transcription processes.
- Biological function summary
NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.
- Pathways
NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.
- Associated diseases and disorders
NASP associates with cancer and infertility. The aberrant expression of NASP is observed in various tumors implying its contribution to tumorigenesis and proliferation. Its association with other histone chaperones such as ASF1 highlights its potential role in oncogenic pathways. Furthermore alterations in NASP expression affect sperm development linking it to male infertility and emphasizing its necessity in reproductive biology.
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1 product image
Sanger Sequencing - Human NASP knockout HEK-293T cell lysate (ab258058)
Homozygous: 27 bp deletion in exon 1
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