Human NASP knockout HEK-293T cell lysate
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NASP KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.
View Alternative Names
5033430J04Rik, AI131596, AI317140, D4Ertd767e, DKFZp547F162, Epcs32, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, MGC93869, NASP_HUMAN, Nuclear autoantigenic sperm protein, PRO1999, RP23 233B9.9, nuclear autoantigenic sperm protein (histone binding)
- Sanger seq
Unknown
Sanger Sequencing - Human NASP knockout HEK-293T cell lysate (AB258058)
Homozygous : 27 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.
Pathways
NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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