Human NCAPD3 (hCAP-D3) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NCAPD3 (hCAP-D3) knockout HeLa cell lysate (AB263273)
Homozygous : 1 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.
Pathways
HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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