Human NCAPG2 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
NCAPG2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 23 bp deletion in exon 3 and 5 bp deletion in exon 3.
View Alternative Names
CAP G2, Chromosome associated protein G2, Condensin 2 complex subunit G2, FLJ20311, LUZP5, Leucine zipper protein 5, MTB, More than blood homolog, Non SMC condensin II complex subunit G2, hCAP G2
- Sanger seq
Unknown
Sanger Sequencing - Human NCAPG2 knockout HEK-293T cell lysate (AB263274)
Allele-2 : 13 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human NCAPG2 knockout HEK-293T cell lysate (AB263274)
Allele-1 : 23 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human NCAPG2 knockout HEK-293T cell lysate (AB263274)
Allele-3 : 5 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The organism relies on NCAPG2 to ensure accurate chromosome segregation. As a component of the condensin II complex it collaborates with other subunits including NCAPD3 and SMC2. The protein's main task is to maintain the structural integrity of chromosomes allowing proper chromosome alignment during mitosis. This integrity is essential for genomic stability reducing the risks of aneuploidy.
Pathways
NCAPG2 is deeply involved in the regulation of the cell cycle. It takes part in both the mitotic prophase and anaphase pathways. Its function relates closely to other proteins like Cdc2 and Cyclin B which direct the progression of cell division. By integrating with structural pathways of chromosomes NCAPG2 becomes an important element in preventing mitotic errors and preserving cellular fidelity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com