Human NCOA2 (KAT13C) knockout HEK-293T cell lysate
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NCOA2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
View Alternative Names
Class E basic helix-loop-helix protein 75, MGC138808, NCOA2_HUMAN, Nuclear receptor coactivator 2, Oncogene FGR, SRC 2, Steroid receptor coactivator 2, TIF 2, Transcriptional intermediary factor 2, bHLHe75, glucocorticoid receptor interacting protein 1, hTIF2, p160
- Sanger seq
Unknown
Sanger Sequencing - Human NCOA2 (KAT13C) knockout HEK-293T cell lysate (AB258530)
Homozygous : 1 bp insertion in exon 5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
KAT13C/NCOA2 serves as part of a larger protein complex that regulates the expression of target genes following ligand binding to nuclear receptors. It acts as a scaffold protein recruiting additional transcriptional machinery to ensure efficient transcriptional activation. This role is critical for the regulation of hormone-responsive genes and in metabolism cell proliferation and differentiation processes.
Pathways
KAT13C/NCOA2 plays a significant role in the steroid hormone receptor signaling pathway and the nuclear hormone receptor signaling pathway. It interacts with other proteins like p160 family members and the CREB-binding protein (CBP) facilitating coordinated gene expression involved in hormone response and metabolism. These interactions position KAT13C/NCOA2 as an important player in fine-tuning cellular responses to external hormonal signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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