Human NCOA7 knockout A549 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
NCOA7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon11 and 35 bp insertion in exon11.
View Alternative Names
140 kDa estrogen receptor associated protein, 9030406N13Rik, ERAP140, ESNA1, Estrogen nuclear receptor coactivator 1, FLJ45605, MGC88425, Nbla00052, Nbla10993, Nuclear receptor coactivator 7, RGD1566426, dJ187J11.3
- Sanger seq
Unknown
Sanger Sequencing - Human NCOA7 knockout A549 cell lysate (AB258988)
Allele-2 : 35 bp insertion in exon11
- Sanger seq
Unknown
Sanger Sequencing - Human NCOA7 knockout A549 cell lysate (AB258988)
Allele-1 : 1 bp deletion in exon11
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NCOA7 influences cellular processes through interactions with nuclear receptors. It forms a complex with these receptors to modulate gene expression. This protein engages in the regulation of genes that are involved in cell development and homeostasis. NCOA7's activity can influence metabolic and signaling processes that are critical for maintaining proper cellular environment and function.
Pathways
NCOA7 associates with important biological pathways including nuclear hormone receptor signaling and lipid metabolism. It interacts with proteins like peroxisome proliferator-activated receptors (PPARs) and estrogen receptors (ERs) in these pathways. These interactions facilitate the regulation of genes involved in lipid storage energy balance and other metabolic functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com