Human NDC1 knockout HeLa cell lysate
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NDC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 2 bp deletion in exon2.
View Alternative Names
FLJ10407, FLJ12556, FLJ34120, NDC1 transmembrane nucleoporin, Nuclear division cycle 1 homolog, TMEM48, Transmembrane protein 48, hNDC1
- Sanger seq
Unknown
Sanger Sequencing - Human NDC1 knockout HeLa cell lysate (AB263275)
Allele-1 : 10 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human NDC1 knockout HeLa cell lysate (AB263275)
Allele-2 : 2 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NDC1 interacts with other nucleoporins to form a functional core within the nuclear pore complex. It plays a critical role in regulating the trafficking of macromolecules between the cytoplasm and nucleus. As part of a dynamic assembly NDC1 ensures that nuclear-cytoplasmic transport processes remain efficient. This protein's interaction with other nucleoporins like NUP53 and NUP93 suggests a scaffold function in pore architecture and assembly.
Pathways
NDC1 integrates into the nucleocytoplasmic transport pathway and is essential in cell cycle regulation. This pathway is important for maintaining cellular homeostasis as it manages the exchange of proteins and RNA. NDC1 works alongside proteins such as NUP205 and Gle1 to facilitate effective transport mechanisms. By supporting such pivotal processes NDC1 helps in ensuring proper cellular functionality and division.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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